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algorithm empiria studio li cor rrid scr 014281 commercial assay  (LI-COR)


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    LI-COR algorithm empiria studio li cor rrid scr 014281 commercial assay
    Algorithm Empiria Studio Li Cor Rrid Scr 014281 Commercial Assay, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/algorithm+empiria+studio+li+cor+rrid+scr+014281+commercial+assay/EMPIRIA+STUDIO+SOFTWARE+LICENSE/10__7554_slash_elife__105978__3-330-59-62
    Average 98 stars, based on 312 article reviews
    algorithm empiria studio li cor rrid scr 014281 commercial assay - by Bioz Stars, 2026-09
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    Antibody Labeling:

    Article Title: Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity
    Article Snippet: DOI: https://doi.org/10.7554/eLife.105978 22 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay, kit NEBuilder HiFi DNA Assembly Cloning Kit NEB E5520S Strain, strain background (Escherichia coli) 5- alpha Competent E. coli (High Efficiency) NEB C2987H Commercial assay, kit T7 RiboMAX Large Scale RNA Production System Promega P1300 Commercial assay, kit PureLink HiPure Plasmid FP Maxiprep kit Invitrogen K210027 Commercial assay, kit Vaccinia Capping System NEB M2080S Commercial assay, kit mRNA Cap 2 ́-O- Methyltransferase NEB M0366S Commercial assay, kit Antarctic Phosphatase NEB M0289S Other NorthernMax- Gly Sample Loading Dye Invitrogen AM8551 Other Lipofectamine MessengerMAX Transfection Reagent Invitrogen LMRNA001 Strain, strain background (M. musculus) C57BL/6J Envigo Cell line (Homo sapiens) tSA201 ECACC 96121229 (RRID:CVCL_2737) Other BioTracker NIR680 Merck SCT112 Chemical compound, drug ML336 Cayman Chemical 9001920 Chemical compound, drug Puromycin Thermo Scientific J67236.XF Other 6- well black glass bottom plate CellVis P06- 1.5H- N Other 24- well black glass bottom plate Grenier Bio- One 662892 Other Live Cell Imaging Solution Invitrogen A59688DJ Other Poly- D- lysine–coated glass- bottom 35 mm dishes MatTek P35GC- 1.5- 14- C Software, algorithm Linear unmixing script for Odyssey M images This paper; Ferguson, 2025 Available at https://github.com/ lariferg/spectral_unmixing Software, algorithm ImageJ NIH RRID:SCR_003070 Other Annexin V- CF800 Biotium 29078 Antibody Anti- cadherin- 11 rabbit polyclonal Affinity Biosciences DF3523 (RRID:AB_2835743) Antibody Anti- Phospho- eIF2α (Ser51) rabbit monoclonal Cell Signaling Technology 3398 (RRID:AB_2096481) Antibody Anti- eIF2α mouse monoclonal Cell Signaling Technology 2103 (RRID:AB_836874) Antibody Anti- PKR rabbit polyclonal Proteintech 18244- 1- AP (RRID:AB_2246451) Antibody Anti-Phospho- eIF4E (S209) rabbit monoclonal Abcam ab76256 (RRID:AB_1523534) Antibody Anti-eIF4E mouse monoclonal Invitrogen MA1- 089 (RRID:AB_2536738) Antibody Anti- fibroblast activation protein mouse monoclonal InVivoMAb BE0374 (RRID:AB_2927511) Antibody Anti- puromycin mouse monoclonal Absolute Antibody 3RH11 (RRID:AB_2620162) Antibody Goat anti- mouse IRDye 800CW secondary LI- COR 926- 32210 (RRID:AB_621842) Continued Continued on next page Lim et al. eLife 2025;14:RP105978. .. DOI: https://doi.org/10.7554/eLife.105978 23 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- rabbit IRDye 800CW secondary LI- COR 926- 32213 (RRID:AB_621848) Antibody Donkey anti- mouse IRDye 680RD secondary LI- COR 926- 68072 (RRID:AB_10953628) Other CellTag 700 LI- COR 926- 41090 Commercial assay, kit DyLight Antibody Labeling Kit Thermo Scientific 53062 Software, algorithm Empiria Studio LI- COR RRID:SCR_014281 Commercial assay, kit LEGENDplex Mouse Anti- Virus Response Panel BioLegend 740621 Software, algorithm Qognit BioLegend Other 3D printed vacuum manifold for LEGENDplex filter plates This paper Available at the NIH 3D Print Exchange under accession number 3DPX- 021388 Commercial assay, kit RNeasy Mini Kit QIAGEN 74104 Commercial assay, kit TaqMan Fast Advanced Cells- to- CT Kit Invitrogen A35377 Commercial assay, kit TaqMan array plates Invitrogen 4413261 Software, algorithm StepOne Software Life Technologies RRID:SCR_014281 Software, algorithm Prism GraphPad RRID:SCR_002798 Continued Plasmid cloning and construct design The commercially available TagGFP2 E3L Simplicon vector (SCR725, Merck) served as the saRNA backbone in this study. .. After removal of the TagGFP2- IRES- E3 sequence, various constructs were generated by combining different elements using restriction digests (FastDigest, Thermo Scientific), overlap extension PCR (Phusion, Thermo Scientific), and HiFi assembly (NEBuilder, New England Biolabs).

    Software:

    Article Title: Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity
    Article Snippet: DOI: https://doi.org/10.7554/eLife.105978 22 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay, kit NEBuilder HiFi DNA Assembly Cloning Kit NEB E5520S Strain, strain background (Escherichia coli) 5- alpha Competent E. coli (High Efficiency) NEB C2987H Commercial assay, kit T7 RiboMAX Large Scale RNA Production System Promega P1300 Commercial assay, kit PureLink HiPure Plasmid FP Maxiprep kit Invitrogen K210027 Commercial assay, kit Vaccinia Capping System NEB M2080S Commercial assay, kit mRNA Cap 2 ́-O- Methyltransferase NEB M0366S Commercial assay, kit Antarctic Phosphatase NEB M0289S Other NorthernMax- Gly Sample Loading Dye Invitrogen AM8551 Other Lipofectamine MessengerMAX Transfection Reagent Invitrogen LMRNA001 Strain, strain background (M. musculus) C57BL/6J Envigo Cell line (Homo sapiens) tSA201 ECACC 96121229 (RRID:CVCL_2737) Other BioTracker NIR680 Merck SCT112 Chemical compound, drug ML336 Cayman Chemical 9001920 Chemical compound, drug Puromycin Thermo Scientific J67236.XF Other 6- well black glass bottom plate CellVis P06- 1.5H- N Other 24- well black glass bottom plate Grenier Bio- One 662892 Other Live Cell Imaging Solution Invitrogen A59688DJ Other Poly- D- lysine–coated glass- bottom 35 mm dishes MatTek P35GC- 1.5- 14- C Software, algorithm Linear unmixing script for Odyssey M images This paper; Ferguson, 2025 Available at https://github.com/ lariferg/spectral_unmixing Software, algorithm ImageJ NIH RRID:SCR_003070 Other Annexin V- CF800 Biotium 29078 Antibody Anti- cadherin- 11 rabbit polyclonal Affinity Biosciences DF3523 (RRID:AB_2835743) Antibody Anti- Phospho- eIF2α (Ser51) rabbit monoclonal Cell Signaling Technology 3398 (RRID:AB_2096481) Antibody Anti- eIF2α mouse monoclonal Cell Signaling Technology 2103 (RRID:AB_836874) Antibody Anti- PKR rabbit polyclonal Proteintech 18244- 1- AP (RRID:AB_2246451) Antibody Anti-Phospho- eIF4E (S209) rabbit monoclonal Abcam ab76256 (RRID:AB_1523534) Antibody Anti-eIF4E mouse monoclonal Invitrogen MA1- 089 (RRID:AB_2536738) Antibody Anti- fibroblast activation protein mouse monoclonal InVivoMAb BE0374 (RRID:AB_2927511) Antibody Anti- puromycin mouse monoclonal Absolute Antibody 3RH11 (RRID:AB_2620162) Antibody Goat anti- mouse IRDye 800CW secondary LI- COR 926- 32210 (RRID:AB_621842) Continued Continued on next page Lim et al. eLife 2025;14:RP105978. .. DOI: https://doi.org/10.7554/eLife.105978 23 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- rabbit IRDye 800CW secondary LI- COR 926- 32213 (RRID:AB_621848) Antibody Donkey anti- mouse IRDye 680RD secondary LI- COR 926- 68072 (RRID:AB_10953628) Other CellTag 700 LI- COR 926- 41090 Commercial assay, kit DyLight Antibody Labeling Kit Thermo Scientific 53062 Software, algorithm Empiria Studio LI- COR RRID:SCR_014281 Commercial assay, kit LEGENDplex Mouse Anti- Virus Response Panel BioLegend 740621 Software, algorithm Qognit BioLegend Other 3D printed vacuum manifold for LEGENDplex filter plates This paper Available at the NIH 3D Print Exchange under accession number 3DPX- 021388 Commercial assay, kit RNeasy Mini Kit QIAGEN 74104 Commercial assay, kit TaqMan Fast Advanced Cells- to- CT Kit Invitrogen A35377 Commercial assay, kit TaqMan array plates Invitrogen 4413261 Software, algorithm StepOne Software Life Technologies RRID:SCR_014281 Software, algorithm Prism GraphPad RRID:SCR_002798 Continued Plasmid cloning and construct design The commercially available TagGFP2 E3L Simplicon vector (SCR725, Merck) served as the saRNA backbone in this study. .. After removal of the TagGFP2- IRES- E3 sequence, various constructs were generated by combining different elements using restriction digests (FastDigest, Thermo Scientific), overlap extension PCR (Phusion, Thermo Scientific), and HiFi assembly (NEBuilder, New England Biolabs).

    Virus:

    Article Title: Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity
    Article Snippet: DOI: https://doi.org/10.7554/eLife.105978 22 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay, kit NEBuilder HiFi DNA Assembly Cloning Kit NEB E5520S Strain, strain background (Escherichia coli) 5- alpha Competent E. coli (High Efficiency) NEB C2987H Commercial assay, kit T7 RiboMAX Large Scale RNA Production System Promega P1300 Commercial assay, kit PureLink HiPure Plasmid FP Maxiprep kit Invitrogen K210027 Commercial assay, kit Vaccinia Capping System NEB M2080S Commercial assay, kit mRNA Cap 2 ́-O- Methyltransferase NEB M0366S Commercial assay, kit Antarctic Phosphatase NEB M0289S Other NorthernMax- Gly Sample Loading Dye Invitrogen AM8551 Other Lipofectamine MessengerMAX Transfection Reagent Invitrogen LMRNA001 Strain, strain background (M. musculus) C57BL/6J Envigo Cell line (Homo sapiens) tSA201 ECACC 96121229 (RRID:CVCL_2737) Other BioTracker NIR680 Merck SCT112 Chemical compound, drug ML336 Cayman Chemical 9001920 Chemical compound, drug Puromycin Thermo Scientific J67236.XF Other 6- well black glass bottom plate CellVis P06- 1.5H- N Other 24- well black glass bottom plate Grenier Bio- One 662892 Other Live Cell Imaging Solution Invitrogen A59688DJ Other Poly- D- lysine–coated glass- bottom 35 mm dishes MatTek P35GC- 1.5- 14- C Software, algorithm Linear unmixing script for Odyssey M images This paper; Ferguson, 2025 Available at https://github.com/ lariferg/spectral_unmixing Software, algorithm ImageJ NIH RRID:SCR_003070 Other Annexin V- CF800 Biotium 29078 Antibody Anti- cadherin- 11 rabbit polyclonal Affinity Biosciences DF3523 (RRID:AB_2835743) Antibody Anti- Phospho- eIF2α (Ser51) rabbit monoclonal Cell Signaling Technology 3398 (RRID:AB_2096481) Antibody Anti- eIF2α mouse monoclonal Cell Signaling Technology 2103 (RRID:AB_836874) Antibody Anti- PKR rabbit polyclonal Proteintech 18244- 1- AP (RRID:AB_2246451) Antibody Anti-Phospho- eIF4E (S209) rabbit monoclonal Abcam ab76256 (RRID:AB_1523534) Antibody Anti-eIF4E mouse monoclonal Invitrogen MA1- 089 (RRID:AB_2536738) Antibody Anti- fibroblast activation protein mouse monoclonal InVivoMAb BE0374 (RRID:AB_2927511) Antibody Anti- puromycin mouse monoclonal Absolute Antibody 3RH11 (RRID:AB_2620162) Antibody Goat anti- mouse IRDye 800CW secondary LI- COR 926- 32210 (RRID:AB_621842) Continued Continued on next page Lim et al. eLife 2025;14:RP105978. .. DOI: https://doi.org/10.7554/eLife.105978 23 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- rabbit IRDye 800CW secondary LI- COR 926- 32213 (RRID:AB_621848) Antibody Donkey anti- mouse IRDye 680RD secondary LI- COR 926- 68072 (RRID:AB_10953628) Other CellTag 700 LI- COR 926- 41090 Commercial assay, kit DyLight Antibody Labeling Kit Thermo Scientific 53062 Software, algorithm Empiria Studio LI- COR RRID:SCR_014281 Commercial assay, kit LEGENDplex Mouse Anti- Virus Response Panel BioLegend 740621 Software, algorithm Qognit BioLegend Other 3D printed vacuum manifold for LEGENDplex filter plates This paper Available at the NIH 3D Print Exchange under accession number 3DPX- 021388 Commercial assay, kit RNeasy Mini Kit QIAGEN 74104 Commercial assay, kit TaqMan Fast Advanced Cells- to- CT Kit Invitrogen A35377 Commercial assay, kit TaqMan array plates Invitrogen 4413261 Software, algorithm StepOne Software Life Technologies RRID:SCR_014281 Software, algorithm Prism GraphPad RRID:SCR_002798 Continued Plasmid cloning and construct design The commercially available TagGFP2 E3L Simplicon vector (SCR725, Merck) served as the saRNA backbone in this study. .. After removal of the TagGFP2- IRES- E3 sequence, various constructs were generated by combining different elements using restriction digests (FastDigest, Thermo Scientific), overlap extension PCR (Phusion, Thermo Scientific), and HiFi assembly (NEBuilder, New England Biolabs).

    Plasmid Preparation:

    Article Title: Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity
    Article Snippet: DOI: https://doi.org/10.7554/eLife.105978 22 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay, kit NEBuilder HiFi DNA Assembly Cloning Kit NEB E5520S Strain, strain background (Escherichia coli) 5- alpha Competent E. coli (High Efficiency) NEB C2987H Commercial assay, kit T7 RiboMAX Large Scale RNA Production System Promega P1300 Commercial assay, kit PureLink HiPure Plasmid FP Maxiprep kit Invitrogen K210027 Commercial assay, kit Vaccinia Capping System NEB M2080S Commercial assay, kit mRNA Cap 2 ́-O- Methyltransferase NEB M0366S Commercial assay, kit Antarctic Phosphatase NEB M0289S Other NorthernMax- Gly Sample Loading Dye Invitrogen AM8551 Other Lipofectamine MessengerMAX Transfection Reagent Invitrogen LMRNA001 Strain, strain background (M. musculus) C57BL/6J Envigo Cell line (Homo sapiens) tSA201 ECACC 96121229 (RRID:CVCL_2737) Other BioTracker NIR680 Merck SCT112 Chemical compound, drug ML336 Cayman Chemical 9001920 Chemical compound, drug Puromycin Thermo Scientific J67236.XF Other 6- well black glass bottom plate CellVis P06- 1.5H- N Other 24- well black glass bottom plate Grenier Bio- One 662892 Other Live Cell Imaging Solution Invitrogen A59688DJ Other Poly- D- lysine–coated glass- bottom 35 mm dishes MatTek P35GC- 1.5- 14- C Software, algorithm Linear unmixing script for Odyssey M images This paper; Ferguson, 2025 Available at https://github.com/ lariferg/spectral_unmixing Software, algorithm ImageJ NIH RRID:SCR_003070 Other Annexin V- CF800 Biotium 29078 Antibody Anti- cadherin- 11 rabbit polyclonal Affinity Biosciences DF3523 (RRID:AB_2835743) Antibody Anti- Phospho- eIF2α (Ser51) rabbit monoclonal Cell Signaling Technology 3398 (RRID:AB_2096481) Antibody Anti- eIF2α mouse monoclonal Cell Signaling Technology 2103 (RRID:AB_836874) Antibody Anti- PKR rabbit polyclonal Proteintech 18244- 1- AP (RRID:AB_2246451) Antibody Anti-Phospho- eIF4E (S209) rabbit monoclonal Abcam ab76256 (RRID:AB_1523534) Antibody Anti-eIF4E mouse monoclonal Invitrogen MA1- 089 (RRID:AB_2536738) Antibody Anti- fibroblast activation protein mouse monoclonal InVivoMAb BE0374 (RRID:AB_2927511) Antibody Anti- puromycin mouse monoclonal Absolute Antibody 3RH11 (RRID:AB_2620162) Antibody Goat anti- mouse IRDye 800CW secondary LI- COR 926- 32210 (RRID:AB_621842) Continued Continued on next page Lim et al. eLife 2025;14:RP105978. .. DOI: https://doi.org/10.7554/eLife.105978 23 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- rabbit IRDye 800CW secondary LI- COR 926- 32213 (RRID:AB_621848) Antibody Donkey anti- mouse IRDye 680RD secondary LI- COR 926- 68072 (RRID:AB_10953628) Other CellTag 700 LI- COR 926- 41090 Commercial assay, kit DyLight Antibody Labeling Kit Thermo Scientific 53062 Software, algorithm Empiria Studio LI- COR RRID:SCR_014281 Commercial assay, kit LEGENDplex Mouse Anti- Virus Response Panel BioLegend 740621 Software, algorithm Qognit BioLegend Other 3D printed vacuum manifold for LEGENDplex filter plates This paper Available at the NIH 3D Print Exchange under accession number 3DPX- 021388 Commercial assay, kit RNeasy Mini Kit QIAGEN 74104 Commercial assay, kit TaqMan Fast Advanced Cells- to- CT Kit Invitrogen A35377 Commercial assay, kit TaqMan array plates Invitrogen 4413261 Software, algorithm StepOne Software Life Technologies RRID:SCR_014281 Software, algorithm Prism GraphPad RRID:SCR_002798 Continued Plasmid cloning and construct design The commercially available TagGFP2 E3L Simplicon vector (SCR725, Merck) served as the saRNA backbone in this study. .. After removal of the TagGFP2- IRES- E3 sequence, various constructs were generated by combining different elements using restriction digests (FastDigest, Thermo Scientific), overlap extension PCR (Phusion, Thermo Scientific), and HiFi assembly (NEBuilder, New England Biolabs).

    Cloning:

    Article Title: Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity
    Article Snippet: DOI: https://doi.org/10.7554/eLife.105978 22 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay, kit NEBuilder HiFi DNA Assembly Cloning Kit NEB E5520S Strain, strain background (Escherichia coli) 5- alpha Competent E. coli (High Efficiency) NEB C2987H Commercial assay, kit T7 RiboMAX Large Scale RNA Production System Promega P1300 Commercial assay, kit PureLink HiPure Plasmid FP Maxiprep kit Invitrogen K210027 Commercial assay, kit Vaccinia Capping System NEB M2080S Commercial assay, kit mRNA Cap 2 ́-O- Methyltransferase NEB M0366S Commercial assay, kit Antarctic Phosphatase NEB M0289S Other NorthernMax- Gly Sample Loading Dye Invitrogen AM8551 Other Lipofectamine MessengerMAX Transfection Reagent Invitrogen LMRNA001 Strain, strain background (M. musculus) C57BL/6J Envigo Cell line (Homo sapiens) tSA201 ECACC 96121229 (RRID:CVCL_2737) Other BioTracker NIR680 Merck SCT112 Chemical compound, drug ML336 Cayman Chemical 9001920 Chemical compound, drug Puromycin Thermo Scientific J67236.XF Other 6- well black glass bottom plate CellVis P06- 1.5H- N Other 24- well black glass bottom plate Grenier Bio- One 662892 Other Live Cell Imaging Solution Invitrogen A59688DJ Other Poly- D- lysine–coated glass- bottom 35 mm dishes MatTek P35GC- 1.5- 14- C Software, algorithm Linear unmixing script for Odyssey M images This paper; Ferguson, 2025 Available at https://github.com/ lariferg/spectral_unmixing Software, algorithm ImageJ NIH RRID:SCR_003070 Other Annexin V- CF800 Biotium 29078 Antibody Anti- cadherin- 11 rabbit polyclonal Affinity Biosciences DF3523 (RRID:AB_2835743) Antibody Anti- Phospho- eIF2α (Ser51) rabbit monoclonal Cell Signaling Technology 3398 (RRID:AB_2096481) Antibody Anti- eIF2α mouse monoclonal Cell Signaling Technology 2103 (RRID:AB_836874) Antibody Anti- PKR rabbit polyclonal Proteintech 18244- 1- AP (RRID:AB_2246451) Antibody Anti-Phospho- eIF4E (S209) rabbit monoclonal Abcam ab76256 (RRID:AB_1523534) Antibody Anti-eIF4E mouse monoclonal Invitrogen MA1- 089 (RRID:AB_2536738) Antibody Anti- fibroblast activation protein mouse monoclonal InVivoMAb BE0374 (RRID:AB_2927511) Antibody Anti- puromycin mouse monoclonal Absolute Antibody 3RH11 (RRID:AB_2620162) Antibody Goat anti- mouse IRDye 800CW secondary LI- COR 926- 32210 (RRID:AB_621842) Continued Continued on next page Lim et al. eLife 2025;14:RP105978. .. DOI: https://doi.org/10.7554/eLife.105978 23 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- rabbit IRDye 800CW secondary LI- COR 926- 32213 (RRID:AB_621848) Antibody Donkey anti- mouse IRDye 680RD secondary LI- COR 926- 68072 (RRID:AB_10953628) Other CellTag 700 LI- COR 926- 41090 Commercial assay, kit DyLight Antibody Labeling Kit Thermo Scientific 53062 Software, algorithm Empiria Studio LI- COR RRID:SCR_014281 Commercial assay, kit LEGENDplex Mouse Anti- Virus Response Panel BioLegend 740621 Software, algorithm Qognit BioLegend Other 3D printed vacuum manifold for LEGENDplex filter plates This paper Available at the NIH 3D Print Exchange under accession number 3DPX- 021388 Commercial assay, kit RNeasy Mini Kit QIAGEN 74104 Commercial assay, kit TaqMan Fast Advanced Cells- to- CT Kit Invitrogen A35377 Commercial assay, kit TaqMan array plates Invitrogen 4413261 Software, algorithm StepOne Software Life Technologies RRID:SCR_014281 Software, algorithm Prism GraphPad RRID:SCR_002798 Continued Plasmid cloning and construct design The commercially available TagGFP2 E3L Simplicon vector (SCR725, Merck) served as the saRNA backbone in this study. .. After removal of the TagGFP2- IRES- E3 sequence, various constructs were generated by combining different elements using restriction digests (FastDigest, Thermo Scientific), overlap extension PCR (Phusion, Thermo Scientific), and HiFi assembly (NEBuilder, New England Biolabs).

    Construct:

    Article Title: Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity
    Article Snippet: DOI: https://doi.org/10.7554/eLife.105978 22 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay, kit NEBuilder HiFi DNA Assembly Cloning Kit NEB E5520S Strain, strain background (Escherichia coli) 5- alpha Competent E. coli (High Efficiency) NEB C2987H Commercial assay, kit T7 RiboMAX Large Scale RNA Production System Promega P1300 Commercial assay, kit PureLink HiPure Plasmid FP Maxiprep kit Invitrogen K210027 Commercial assay, kit Vaccinia Capping System NEB M2080S Commercial assay, kit mRNA Cap 2 ́-O- Methyltransferase NEB M0366S Commercial assay, kit Antarctic Phosphatase NEB M0289S Other NorthernMax- Gly Sample Loading Dye Invitrogen AM8551 Other Lipofectamine MessengerMAX Transfection Reagent Invitrogen LMRNA001 Strain, strain background (M. musculus) C57BL/6J Envigo Cell line (Homo sapiens) tSA201 ECACC 96121229 (RRID:CVCL_2737) Other BioTracker NIR680 Merck SCT112 Chemical compound, drug ML336 Cayman Chemical 9001920 Chemical compound, drug Puromycin Thermo Scientific J67236.XF Other 6- well black glass bottom plate CellVis P06- 1.5H- N Other 24- well black glass bottom plate Grenier Bio- One 662892 Other Live Cell Imaging Solution Invitrogen A59688DJ Other Poly- D- lysine–coated glass- bottom 35 mm dishes MatTek P35GC- 1.5- 14- C Software, algorithm Linear unmixing script for Odyssey M images This paper; Ferguson, 2025 Available at https://github.com/ lariferg/spectral_unmixing Software, algorithm ImageJ NIH RRID:SCR_003070 Other Annexin V- CF800 Biotium 29078 Antibody Anti- cadherin- 11 rabbit polyclonal Affinity Biosciences DF3523 (RRID:AB_2835743) Antibody Anti- Phospho- eIF2α (Ser51) rabbit monoclonal Cell Signaling Technology 3398 (RRID:AB_2096481) Antibody Anti- eIF2α mouse monoclonal Cell Signaling Technology 2103 (RRID:AB_836874) Antibody Anti- PKR rabbit polyclonal Proteintech 18244- 1- AP (RRID:AB_2246451) Antibody Anti-Phospho- eIF4E (S209) rabbit monoclonal Abcam ab76256 (RRID:AB_1523534) Antibody Anti-eIF4E mouse monoclonal Invitrogen MA1- 089 (RRID:AB_2536738) Antibody Anti- fibroblast activation protein mouse monoclonal InVivoMAb BE0374 (RRID:AB_2927511) Antibody Anti- puromycin mouse monoclonal Absolute Antibody 3RH11 (RRID:AB_2620162) Antibody Goat anti- mouse IRDye 800CW secondary LI- COR 926- 32210 (RRID:AB_621842) Continued Continued on next page Lim et al. eLife 2025;14:RP105978. .. DOI: https://doi.org/10.7554/eLife.105978 23 of 41 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti- rabbit IRDye 800CW secondary LI- COR 926- 32213 (RRID:AB_621848) Antibody Donkey anti- mouse IRDye 680RD secondary LI- COR 926- 68072 (RRID:AB_10953628) Other CellTag 700 LI- COR 926- 41090 Commercial assay, kit DyLight Antibody Labeling Kit Thermo Scientific 53062 Software, algorithm Empiria Studio LI- COR RRID:SCR_014281 Commercial assay, kit LEGENDplex Mouse Anti- Virus Response Panel BioLegend 740621 Software, algorithm Qognit BioLegend Other 3D printed vacuum manifold for LEGENDplex filter plates This paper Available at the NIH 3D Print Exchange under accession number 3DPX- 021388 Commercial assay, kit RNeasy Mini Kit QIAGEN 74104 Commercial assay, kit TaqMan Fast Advanced Cells- to- CT Kit Invitrogen A35377 Commercial assay, kit TaqMan array plates Invitrogen 4413261 Software, algorithm StepOne Software Life Technologies RRID:SCR_014281 Software, algorithm Prism GraphPad RRID:SCR_002798 Continued Plasmid cloning and construct design The commercially available TagGFP2 E3L Simplicon vector (SCR725, Merck) served as the saRNA backbone in this study. .. After removal of the TagGFP2- IRES- E3 sequence, various constructs were generated by combining different elements using restriction digests (FastDigest, Thermo Scientific), overlap extension PCR (Phusion, Thermo Scientific), and HiFi assembly (NEBuilder, New England Biolabs).



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    algorithm empiria studio li cor rrid scr 014281 commercial assay  (LI-COR)
    98
    LI-COR algorithm empiria studio li cor rrid scr 014281 commercial assay
    Algorithm Empiria Studio Li Cor Rrid Scr 014281 Commercial Assay, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/algorithm+empiria+studio+li+cor+rrid+scr+014281+commercial+assay/EMPIRIA+STUDIO+SOFTWARE+LICENSE/10__7554_slash_elife__105978__3-330-59-62
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    algorithm empiria studio li cor rrid scr 014281 commercial assay - by Bioz Stars, 2026-09
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